Burn Wound Closure With Cultured Autologous Keratinocytes
نویسنده
چکیده
Improved survival of patients with extensive burn injuries requires the development of better techniques for timely and permanent closure of the full-thickness wound. We developed procedures for establishing confluent, stratified layers of cultured, autologous keratinocytes on the surface of a modified collagen-glycosaminoglycan membrane that contains autologous fibroblasts. We transferred the composite grafts onto limited areas of excised full-thickness burn wounds of four patients with extensive burn injuries. Our results indicate that this composite graft material has acceptable "take," with formation of a basement membrane within 9 days of graft placement. Use of this technique formajorwound coverage may offer a significant advance in the care of extensively burned patients and also may be useful for providing skin for reconstructive surgery. (JAMA. 1989;262:2125-2130)
منابع مشابه
Effect of Sprayed Cultured Autologous Keratinocyte Suspension Used Alone and in Combination with Fibrin Glue to Closure of 3rd Degree Burn Wounds in
Objective- Our point is to determine if the treatment of the 3rd degree burn wounds makes any difference if sprayed cultured autologous keratinocyte used alone or in combination with Fibrin glue. Design- Animal experimental study. Animals- 30 male Wistar rats. Procedures- Thirty male Wistar rats were selected and their keratinocytes were isolated and cultured from small skin biops...
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Cultured autologous keratinocytes (CAK) have been heralded as a means to achieve more rapid closure of massive burn wounds. Despite the claimed benefits of this technology, we have failed to identify its positive impact on wound closure in extensively burned patients. Sixteen patients with a mean age of 29.7 years (range, 10-56 years) and a mean total body surface area burn of 68.2% (range, 42%...
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